The genus Weissella: taxonomy, ecology and biotechnological potential. The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. The values of Mark-Houwink parameters could be universalized with certain precautions, being an indication for the calculation of molecular weight in the temperature range of 20-40C. A similar combination involves the intrinsic viscosity and specific volume: (a/b) is called Einstein viscosity increment, and Vs is specific volume (cm3/g). 4D imaging reveals mechanisms of clay-carbon protection and The fast increasing of these polyglucosans for medical, industrial and research purposes motivated a survey of the types obtainable. Dextrans in Aqueous Solution. 1905/05) for their financial support. Pore closure examination was performed using an optical microscope (BX43, Olympus, Japan) immediately (time 0), 4, 8, and 24h following device application. 56 The linkage that the enzyme dextranase breaks down is indicated by the black arrow. Chem. Ahn, G.Y., Eo, HS., Kim, D. et al. In general, Mv is not experimentally accessible, whereas other average molecular weights are accessible. Viscosity of in water solution polysaccharides depends on intrinsic characteristics of the biopolymer (such as molecular weight, volume, size, shape, surface charge, deformation facility, esterification degree, and galacturonic content) and on ambient factors (such as pH, temperature, ionic strength, solvent, etc.). Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. WebAmines per 70KDa dextran Theeffectofchargerepulsiononthe hydrodynamicradiuscanbeseenwithRh increaseswithchargedensity.Charge repulsioncausesthehydrodynamicradius toincrease.Withincreasingcharge,the dextranprogressivelyunfolded.Evena lowlevelofaminationcausesthepolymer tounfold. Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine WebDextran produced from Weissella cibaria JAG8 was purified and characterized. Sung-Wook Choi. Gekko, K. (1971). In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). This conjecture found to be correct. With FITC-dextran 20 K, cumulative Various average molecular weights (Mv, Mn, Mw, and Mz) instead of Mv were substituted in M-H equation. An official website of the United States government. After microporation, the TEWL values of the human cadaver skin increased from 11.30.6 to 33.01.0, 10.71.5 to 45.31.5, and 10.70.6 to 61.02.0g/m2/h when the microporation was performed 2, 5, and 10 times, respectively. These studies were carried out at the Hisko (Gunpo, Korea). After 24h the micropores were either closed or reduced further to smaller sizes, 37.5020.41, 50.5718.07, and 58.5814.99m, for 2, 5, and 10 times microporation, respectively. osmotic pressure or light scattering). Optical properties of dextran in solution and in films. (2003). Journal Non-Newtonian Fluid Mech., 97, 295-301. In vivo iontophoretic delivery of salmon calcitonin across microporated skin. Lucifer yellow (LY) and FITC-labelled dextran stock solutions were prepared in Ringers-HEPES buffer (150 mM NaCl, 3.4 mM CaCl 2, 1.2 mM MgCl 2, 5.2 mM KCl, 0.5 mM NaHCO 3, 2.8 mM glucose, and 10 mM HEPES) and frozen at 20C. Human skins (Full-thickness human skin) were supplied by HansBiomed. Int J Pharm Investig. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). 2006;3(3):37385. Carbohydrate Polymers, 36, 319-326. Thomas Gutberlet (Lipoteichoic Acid): Statistiques d'influence official website and that any information you provide is encrypted WebTry out PMC Labs and tell us what you think. Solvent effects on polysaccharide conformation. Thomas Gutberlet (Lipoteichoic Acid): Statistiques d'influence Anal. Ed., 9(2), 85-90. Synthesis of dextran by dextransucrase treated, sucrose-supplemented skimmed milk revealed the promising potential of dextran as a food additive. Figure 2 shows the classical method for calculating Mark-Houwink parameters, where a linear relation between the intrinsic viscosity and the molecular weight is established for each temperature. where tr is the column retention time in minutes (2 of 0.9792). The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. Pavlov, G.M. Challenges in analysis of high-molar mass dextrans: Comparison of HPSEC, AsFlFFF and DOSY NMR spectroscopy. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter (d h ~ 70 nm) on order of the collagen mesh size. where c is the solute concentration (g/cm3), [] is the intrinsic viscosity (cm3/g), and kH represents Huggins constants (cm3/g). Recent Pat Drug Deliv Formul. Pharm Res. Drug deliv. The Mark-Houwink parameters is calculated molecular weights range from 8.8 to 200kDa. These studies on Mark-Houwink parameters are usually carried out at a given temperature, obtaining a consistent result but in a very limited temperature range [68]. Kano S, Todo H, Furui K, Kenichi S, Tokudome Y, Hashimoto F, Kojima H, Sugibayashi K. Comparison of several reconstructed cultured human skin models by microscopic observation: their usefulness as an alternative membrane for skin in drug permeation experiments. A long-chain polymer molecule in solution takes on a somewhat kinked or curled shape, intermediate between a tightly curled mass (coil) and a rigid linear configuration. Where f0 is the frictional coefficient of a sphere having the same hydrodynamic (hydrated or solvated) volume V as the particle. In addition, the permeation rate was higher in pig skin than in human skin. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Transdermal drug delivery: overcoming the skins barrier function. As well as being able to measure hydrodynamic radius in the Zetasizer (if you have access to one), it's also provided with a calculator that allows The cumulative permeation of FITC-dextran 10K was 4.820.32M, in the human skin and 5.920.46M in the pig skin. Transdermal drug delivery system for non-steroidal anti inflammatory drugs: a review. The dextran was chosen as another branched polysaccharide which, like the others was suspected to show hyperbranched behavior. The Mark-Houwink parameters and the effect of temperature on them are specifically studied. hydrodynamic Journal of Applied Polymer Science, 72, 871-876. SWC designed and coordinated the research. Kumar SV, Tarun P, Kumar TA. 2012;9(2):14754. The intrinsic viscosity calculation requires several concentrations, and because of this inconvenient will not use the Huggins method in this paper. Dextran standards with sequence molecular weight from 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa were utilized for the plot of calibration curve. Structural and biocompatibility properties of dextran from Structural and biocompatibility properties of dextran from When applied to adherent cell cultures, the APGD-t is capable of inducing temporary cell permeabilization. (2008). Therefore, the trend of "a" and kM-H decreases with T, where the presence of ethylene glycol or dimethylsulfoxide strongly changes the interactions of this solvent with the macromolecule. Kasaai, Mohammad R. (2012). Regulating dynamic signaling between hematopoietic stem cells WebThe Stokes radius or StokesEinstein radius of a solute is the radius of a hard sphere that diffuses at the same rate as that solute. Hydrodynamic Properties of Tragacanthin. Determination of intrinsic viscosities of macromolecules and nanoparticles. Transdermal delivery is of great importance for the effective delivery of bioactive or therapeutic agents into a body. Format), Citation-(EndNote
(2003). However, when molecules deviate from globular to non-spherical or elongated structures then Rg/Rh tend to values upwards of With FITC-dextran 20K, cumulative permeation in the human skin was 2.530.48M, and 3.340.19M in the pig skin, which significantly lower than FITC-dextran 4K. Cumulative amount of FITC-dextran permeated after microporation on the human and pig skin for FITC-dextran M.W.. a M.W. Learn More. Search ; Rjumtsev, E.I. Just in case anyone wants to use a different molecular weight to estimate the hydrodynamic size: the calculator is integrated into the classic Zeta Hydrodynamic 2016;9706:97061E6. Amersham Biosciences, AA Edition, www.amershambiosciences.com. Elias, Hans-Georg (1975). dextran hydrodynamic radius. Critical parameters for gel permeation dextran RBECs were passaged onto cell culture inserts as described and cultured for a further 714 days in vitro. The slope is a value and intercept is equal to the ln kMH value [47]. Provided by the Springer Nature SharedIt content-sharing initiative. Mainaa, Ndegwa Henry; Pitknen, Leena; Heikkinen, Sami; Tuomainen; Pivi; Virkki, Liisa; Tenkanen, Maija (2014). Advances in Carbohydrate Analysis, 1, 63-144. Not for use in diagnostic procedures. Multiple Linear Least-Squares Fits with a Common Intercept: Determination of the Intrinsic Viscosity of Macromolecules in Solution. The mechanism of the dextran-induced red blood cell Aggregation. CAS Small Intestinal Permeability and Gut-Transit Time Determined However, the currently available transdermal systems are limited by low drug permeability across the skin due to the lipophilic barrier function of the stratum corneum (SC), the outermost skin layer of the skin [3,4,5]; the methods favor low molecular mass, and lipophilic molecules, making it difficult to exploit the transdermal route for large and water-soluble molecules such as proteins and peptides. Palmuti Braga Vettoria, Mary Helen; Martins Franchetti, Sandra Mara; Contiero, Jonas (2012). where (poise) is the viscosity, the (g/cm3) fluid density, and t the liquid draining time (s) subscript zero is referred to solvent, and without subscript is referred to the solution [40], and inherent viscosity is i = r -1. Its Invariancy with Respect to the Cross-Sectional Dimensions of Polymer Chains. Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). Journal of Physical Chemistry. (1954). Biomater Res 24, 22 (2020). The hydrodynamic radius R h, defined as the radius of a hard sphere that diffuses at the same rate as the solute, takes these effects into account. From Table 1, It is worth noting that the value of 2 is above 0.95, being an acceptable value for viscosimetric measurement. The Transdermal diffusion cell system was protected from light. Privacy The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. Viet, D.; Beck-Candanedo, S.; Gray D. G. (2008). Mitragotri S. Mechanical disruption of skin barrier for vaccine delivery. For a rigid or rod like polymer molecule that is greatly extended in solution, the Mark-Houwink a constant approaches to a value of 2.0 [48].The hydrodynamic radius (RH), for a sphere ((a/b) = 2.5) is given by the Einstein relation [49]. Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. Arond L.H. The enhanced permeability through micropores was confirmed by Franz diffusion cell. The formula is verified for different systems of polymer-solvent and the values are in accord with those obtained by extrapolation. (1954). Microneedles and their applications. Soc., 16, 3A, 426-433. Journal of Applied Polymer Science, 13, 2031-2032. FITC-dextran solution (600L) was added to the donor chamber. The Intrinsic Viscosity of Biological Macromolecules. Conversely, fluorescein sodium was the compound selected to model hydrophilic, low MW drugs. 4K, 10K, and 20K) permeated through the human, and pig skin after microporation.